A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen
Article Abstract:
A screen based on high-content imaging is developed to test the utility of the library for arrayed screens in order to identify genes required for mitotic progression in human cancer cells and applied to an arrayed set of 5,000 unique short hairpin RNA (shRNA)-expressing lentiviruses that target 1,028 human genes. The screen identified several known and ~100 candidate regulators of mitotic progression and proliferation.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
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INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair
Article Abstract:
The role played by ATP-dependant chromatin remodeling in transcription as well as in chromatin remodeling and DNA repair is examined. It is found that INO80 chromatin remodeling complex participates in the repair of double-strand break in yeast and findings suggest that an ATP-dependant chromatin remodeling complex can efficiently read a DNA repair histone code.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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Exploration of essential gene functions via titratable promoter alleles
Article Abstract:
Essential yeast genes were subjected to morphological analysis, size profiling, drug sensitivity screening and microarray expression profiling for determining the essential gene functions. The study provides a resource to complement the yeast knockout collection and the automated genetic analysis confirms the compatibility of the yeast strains.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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