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Biological sciences

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A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

Article Abstract:

A simple procedure for the subtractive hybridization isolation of DNA sequences specific to Xylella fastidiosa from diseased citrus plants was conducted. The procedure, based on the technique described by A.P. Monaco, S.A. Latt, H.D. Ochs, W. Middlesworth and M. Kunkel, resulted in the detection of the bacteria even in citrus plants that do not exhibit the disease. The isolation of Xy. fastidiosa-specific DNA from citrus shows that subtractive hybridization can be applied to other microorganisms.

Plant-pathogen relationships, Bacteria, Phytopathogenic, Phytopathogenic bacteria, Bacterial diseases of plants, Bacterial plant diseases

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Evidence for metabolism of o-xylene by simultaneous ring and methyl group oxidation in a new soil isolate

Article Abstract:

An o-xylene-utilizing Rhodococcus, strain B3, was isolated. Results of simultaneous adaption experiments, fortuitous oxidation studies and studies of product formation by a mutant revealed that pathways for both ring and methyl group oxidation can operate in the same microorganism. It was also found that the ring oxidation pathways can be initiated by a monooxygenase-catalyzed reaction.

Adaptation (Biology), Evolutionary adaptation, Oxidation-reduction reaction, Oxidation-reduction reactions, Methyl groups, Methyl compounds

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The role of sulfoacetaldehyde sulfo-lyase in the mineralization of isethionate by an environmental Acinobacter isolate

Article Abstract:

The mineralization of isethionate by an environmental Acinetobacter isolate was reported. Isethionate with concentration up to 20mM was used as carbon and energy source. Sulfite-oxidizing activity were observed in isethionate-grown cells of Acinetobacter sp. ICD. Results reveal that sulfoacetaldehyde sulfo-lyase plays a significant role in the mineralization of biogenic sulfonates.

Biomineralization, Sulfites

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