A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905

Article Abstract:

A Bacillus sphaericus monooxygenase capable of catalyzing the first two steps in the degradation of p-nitrophenol (PNP) via 4-nitrocatechol and 1,2,4-trihydroxybenzene (THB) was identified and characterized. Nitrite was produced stoichiometrically by PNP-grown cells. Results of the THB stimulation of oxygen consumption reveal that THB acts as a substrate for a ring cleavage dioxygenase. It was also observed that a single monooxygenase system was capable of catalyzing the initial hydroxylation of PNP and the oxidative release of the nitro group from the 4-nitrocatechol.

Methods, Biodegradation, Phenols, Phenols (Class of compounds), Bacillus (Bacteria), Bacillus, Benzene

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Differences in virulence among Escherichia coli O157:H7 strains isolated from humans during disease outbreaks and from healthy cattle

Article Abstract:

Gnotobiotic piglet model is used for investigating the virulence of isolates from healthy cattle and from humans in disease outbreaks and also for determining the correlation between production of Shiga toxin 1 (Stx1) and Stx2 and virulence. The studies have shown that disease outbreaks select for producers of high levels of Stx2 among Escherichia coli O157:H7 strains shed by animals and that Stx1 expression is not important in human outbreaks.

Science & research, Evaluation, Genetic aspects, Escherichia coli, Virulence (Microbiology)

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