Development of an in vitro bioassay for Clostridium botulinum type B neurotoxin in foods that is more sensitive than the mouse bioassay

Article Abstract:

The development of an in vitro bioassay for Clostridium botulinum type B neurotoxin in foods that is more sensitive than the mouse bioassay is described. The enzymic activity of the neurotoxin's light chain was used to amplify the assay which conducted in three stages. Results indicate that the endopeptidase assay can differentiate between the type B neurotoxins produced by proteolytic and nonproteolytic strains of Clostridium botulinium type B.

Author: Taylor, Richard, Wictome, Matthew, Newton, Kirsti, Jameson, Karen, Hallis, Bassam, Dunnigan, Paul, MacKay, Eric, Clarke, Sally, Gaze, Joy, Foster, Keith, Shone, Clifford
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Mice, Mice (Rodents), Clostridium botulinum, Neurotoxic agents

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Real-time PCR assay for detection and enumeration of Dekkera bruxellensis in wine

Article Abstract:

A real time or quantitative PCR (QPCR) assay is developed for the detection and quantification of D. bruxellensis in wine. The method is helpful to identify possible routes of D. bruxellensis infection in winery environments.

Author: Phister Trevor G., Mills Dvid A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Science & research, Quantitative genetics

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Subjects list: Research, Biological assay, Bioassay
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