Altered metabolic flux due to deletion of odhA causes L-glutamate overproduction in corynebacterium glutamicum

Article Abstract:

A study was conducted to analyze the relationship between L-glutamate-producing induction, alterations in the fatty acid composition of the membrane, 2-oxoglutarate dehydrogenase complex activity, changes in metabolic flux, and L-glutamate production. The findings suggest that optimizing the metabolic flux from 2-oxoglutarate to L-glutamate by tuning glutamate dehydrogenase activity increased the L-glutamate production of the odhA-deleted strain.

Analysis, Enzymes, Bacterial growth, Enzyme synthesis, Glutamate dehydrogenase

---

Functional analysis of the twin-arginine translocation pathway in Corynebacterium glutamicum ATCC 13869

Article Abstract:

The tatA, tatB, tatC, and tatE genes from Corynebacterium glutamicum ATCC 13869 is cloned and mutants carrying deletions of each tat gene or of both the tatA and tatE genes is constructed to assess the function of the C. glutamicum Tat pathway. Unlike the TatB proteins from gram-negative bacteria C. glutamicum TatB is dispensable for Tat function, although it is required for maximal efficiency of secretion.

France, Bacterial genetics

---

Quantitative detection of Corynebacterium casei in cheese by real-time PCR

Article Abstract:

A SYBR green I real-time PCR method is developed to quantify Corynebacterium casei, a major species of smear-ripened cheeses, using primers designed to target the 16S rRNA gene. Results reveal that SYBR green I real-time PCR might be used as a reliable species-specific method for quantification of bacteria from the surface of cheeses.

Ribosomal RNA

Similar abstracts:

• Abstracts: Reduced folate supply as a key to enhanced L-serine production by Corynebacterium glutamicum
• Abstracts: Metabolic engineering of Lactobacillus plantarun for production of L-ribulose. Cloning and expression of a xylitol-4-dehydrogenase gene from Pantoea ananatis
• Abstracts: Reduced glutathione levels affect the culmination and cell fate decision in Dictyostelium discoideum
• Abstracts: Nested PCR for ultrasensitive detection of the potato ring rot bacterium, Clavibacter michiganensis subsp. sependonicus
• Abstracts: Genetic segregation of microsatellite markers in Saccharum officinarum and S. spontaneum. Differential chromosome pairing affinities at meiosis in polyploid sugarcane revealed by molecular markers

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.