An alkalophilic Bacillus sp. produces 2-phenylethylamine

Article Abstract:

Cells of alkalophilic Bacillus sp. strain YN-2000 produced a large amount of 2-phenylethylamine which is secreted in the medium during cell growth. Variations in the pH of the medium cause a change in the amounts of 2-phanylethylamine in the cells as well as the medium. Strain YN-2000 is used for the industrial production of 2-phenylethylamine which is the raw material for chemical synthesis of several medicines. Examination of the distribution of polyamines in microorganisms under extreme conditions revealed an unidentified basic compound in an extract of alkalophilic Bacillus sp. strain YN-2000 (3,5).

Analysis, Phenethylamines

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Alkalophilic Bacillus sp. strain LG12 has a series of serine protease genes

Article Abstract:

The Bacillus sp. strain LG12 contains the sprA, sprB, sprC, and sprD subtilisin-like protease genes which contain propeptides and signal sequences suggestive of secreted proteins. The SprC and SprD proteases are not related to subtilisin BPN' and other subtilisins. The downstream sprC and sprD genes seem to be transcribed independently while the upstream sprA and sprB genes seem to be a part of the same transcript. The signal sequence of SprB and the mature sequence of SprC contain one cysteine each while SprA and SprD contain no cysteine.

Observations, Amino acid sequence, Amino acid sequencing

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Buffering capacity and membrane H+ conductance of neutrophilic and alkalophilic gram-positive bacteria

Article Abstract:

Research was conducted to examine buffering capacity and membrane H+ conductance in three gram-positive bacteria, namely, Staphylococcus aureus,, Bacillus alcalophilus and Bacillus subtilis. Measurement of both parameters were done using an acid pulse technique which gave reproducible measurements over a wie range of external pHs. Results indicate that the buffering capacity and membrane H+ conductance of B alcalophilus are influence by the pH of the medium and culture conditions.

Staphylococcus aureus, Bacillus subtilis

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