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Fine structure of E. coli RNA polymerase-promoter interactions: alpha subunit binding to the UP element minor groove

Article Abstract:

Fine structure of Escherichia coli RNA polymerase (RNAP)-promoter interactions is discussed with proposal of a model in which the alpha subunit contacts UP element DNA through amino acid residues in two helix-hairpin-helix motifs. Information about recognition of the core promoter by sigma(super.70) has been extended by identification of functional groups in the major grooves of the -10 and -35 hexamers in which modifications interfere with RNAP binding. Footprinting studies of RNAP on templates with the rrnB P1 core promoter fused to UP elements of one proximal subsite were carried out. The UP element is a little upstream of the -35 hexamer.

Author: Ross, Wilma, Ernst, Alexander, Gourse, Richard L.
Publisher: Cold Spring Harbor Laboratory Press
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2001
Bacteria, Cellular signal transduction, Promoters (Genetics), RNA, Binding sites (Biochemistry), Active sites (Biochemistry)

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An intersubunit contact stimulating transcription initiation by E. coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4

Article Abstract:

Research has been conducted on C-terminal domain of Escherichia coli RNA polymerase alpha subunit. The authors have investigated specific substitutions in sigma region and in alpha subunit, and report that these substitutions decrease transcription initiation from promoters containing some upstream element DNA.

Author: Ross, Wilma, Gourse, Richard L., Schneider, David A., Paul, Brian J., Mertens, Aaron
Publisher: Cold Spring Harbor Laboratory Press
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2003
Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Analysis, Developmental biology, Genetic transcription, Transcription (Genetics), Genetic research

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rRNA promoter regulation by nonoptimal binding of sigma region 1.2: An additional recognition element for RNA polymerase

Article Abstract:

A study reports an unknown interaction with Escherichia coli ribonucleic acid polymerase (RNAP) that defines an additional recognition element in bacterial promoters. Results indicate that ribosomal RNA (rRNA) promoters create the short-lived complex required for specific responses to the RNAP binding factors ppGpp and DksA by making a non-optimal sigma1.2-discriminator interaction ultimately accounting for regulation of ribosome synthesis.

Author: Ross, Wilma, Gourse, Richard L., Haugen, Shanil P., Berkmen, Melanie B., Gaal, Tamas; Ward, Christopher
Publisher: Elsevier B.V.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
Science & research, Genetic regulation, Ribosomal RNA

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Subjects list: Research, United States, Physiological aspects, Genetic aspects, DNA, Escherichia coli, RNA polymerases
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