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Application of a vital fluorescent staining method for simultaneous, near-real-time concentration monitoring of two bacterial strains in an Atlantic coastal plain

Article Abstract:

Two field experiments are conducted in consecutive years (2000 and 2001) for which two strains of bacteria were labeled with vital fluorescent stains, coinjected, and tracked in a shallow coastal aquifer. The results relate specifically to the ability to simultaneously track both bacterial strains through the subsurface in near-real time and to show that microbial properties were the main determinant in the control of transport.

Author: Hall, James A., Johnson, William P., Fuller, Mark E., Streger, Sheryl H., Mailloux, Brian J., Onstott, Tullis C., Vainberg, Simon, Zhang, Pengfei, Kovacik, William P.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Science & research, Microbiological research

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Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments

Article Abstract:

Research describes development of a fluorescent labeling technique for bacteria in which the fluorophore does not affect cell transport or viability. Data reveal 5-(And 6-)-carboxyfluorescein diacetate, succinimidyl ester is an efficient and physiologically suitable label for investigating bacterial transport studies.

Author: Hall, James A., Balkwill, David L., Fuller, Mark E., Streger, Sheryl H., Rothmel, Randi K., Mailloux, Brian J., Onstott, Tullis C., Fredrickson, James K., DeFlaun, Mary F.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
United States, Statistical Data Included, Analysis, Usage, Measurement, Physiological aspects, Fluorescence, Biological transport, Fluorescence spectroscopy

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Archaeal diversity in waters from deep South African gold mines

Article Abstract:

Researchers have discovered archaeal communities in South African gold mines that are completely distinct from those found in other environments. The communities were phylogenetically diverse, with most members related to uncultivated species.

Author: Onstott, Tullis C., Fredrickson, James K., Takai, Ken, Moser, Duane P., DeFlaun, Mary
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Archaeabacteria, Mineral deposits, Mines and mineral resources, Mining, Archaea

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Subjects list: Research, Bacteria
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