Assembly of functionally active Drosophila origin recognition complex from recombinant proteins

Article Abstract:

In eukaryotes initiation sites of chromosomal DNA replication are thought to be determined partly by binding of a heteromeric origin recognition complex (ORC) to DNA. Genes encoding subunits of Drosophila ORC have been cloned. Each gene is unique and can be mapped to a discrete chromosome location. This would indicate that the pattern and developmental regulation of origin usage in Drosophila might not be regulated by a large family of different ORC proteins alone. The 6-subunit ORC can be put back together with recombinant proteins into a complex that restarts DNA replication in ORC-depleted Xenopus or Drosophila egg extracts.

United States, Usage, DNA, Genetic recombination, Chromosome mapping, Xenopus

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members only encodes a Drosophila nucleoporin required for Rel protein import and immune response activation

Article Abstract:

The Drosophila gene members only (mbo) has been found to encode a nucleoporin necessary for Rel protein import and immune-response activation. The nucleoporin is homologous to the mammalian Nup88. Study results show certain nuclear import events require different nucleoporins in vivo and suggest mbo has a regulatory function in signal transduction.

Sweden, Statistical Data Included, Abnormalities, Physiological aspects, Carrier proteins, Transport proteins, Cellular signal transduction, Immunocytochemistry, Cell nuclei, Cell nucleus, Nuclear membranes, Nuclear membrane, Immune response, Trachea

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