Candida albicans adherence to a human oesophageal cell line

Article Abstract:

Candida albicans is a fungal pathogen that causes infection via cell surface adhesins. In AIDS patients, esophageal candidiasis is established by recognition of esophageal epithelial cells by C. albicans. To determine the mechanism of this interaction, the human epithelial esophageal cell line, HET1-A, was cultured with C. albicans grown on D-galactose or on D-glucose. In vitro adherence assay revealed greater adherence of cells grown in D-galactose than in D-glucose. D-glucosamine or N-acetyl-D-glucosamine inhibited adhesion of C. albicans to HET1-A cells. SDS-PAGE and immunoblotting showed that adherence of C. albicans to HET1-A was influenced by cell surface proteins.

Microorganisms, Microbial adhesion, Cell adhesion

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Disruption studies of a Candida albicans gene, ELF1: a member of the ATP-binding cassette family

Article Abstract:

A new member of the ATP-binding cassette gene family of Candida albicans and its gene disruption ability were identified. Results showed that the gene family includes both membrane transport proteins which confered a drug resistance phenotype and proteins whose functions are associated with protein translation. It was also observed that elongation like factor displayed the greatest homology with S. cerevisiae ORF and lower homology with fungal elongation factor.

Genetic aspects, Carrier proteins, Transport proteins, Drug resistance in microorganisms, Microbial drug resistance, Membrane proteins, Adenosine triphosphate, ATP

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Flocculation of hyphae is associated with a deletion in the putative CaHK1 two-component histidine kinase gene from Candida albicans

Article Abstract:

A study was conducted to analyze identify genes of enviornmentally regulated pathways by searching for histidine kinase homologues of Candida albicans. C. albican strains were prepared either in YPD complex medium or YNB minimal medium at 28 degrees C. Results indicated that delta-cahk1 mutants flocculate extensively in a gene-dosage-dependent manner under conditions which induce germ-tube formation.

Histidine

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