Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples

Article Abstract:

A study was conducted to compare the effects of known polymerase chain reaction (PCR)-inhibiting samples on nine thermostable DNA polymerases. Samples of blood, cheese, feces and meat as well as various ions were added to PCR mixtures containing various thermostable DNA polymerases. Th eDNA polymerase from Thermotoga maritima was found to be the most susceptible to PCR inhibitors present in cheese, feces and meat samples. Results suggested that the PCR-inhibiting effect of various components in biological samples can be eliminated to some extent by the use of appropriate DNA polymerase.

Enzymes, Polymerase chain reaction, DNA polymerases

---

Multiple alpha-glucoside transporter genes in brewer's yeast

Article Abstract:

Research was conducted to examine the presence of maltose and maltotriose transporter gene sequences in brewer's yeast. To examine the alleles of maltose and maltotriose transporter genes, 30 brewing strains of yeast, which include five ale strains and 25 lager strains, were studied. Results revealed the presence of alleles of known alpha-glucoside transporter genes. Findings also demonstrated that related sequences map to other chromosomes in some of the genomes examined.

Genetic aspects, Bacterial genetics, Genomes, Yeast, Yeast (Food product)

---

Similar abstracts:

• Abstracts: Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein. Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism
• Abstracts: RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli. Rickettsia prowazekii sigma factor sigma-73 can be overexpressed in Escherichia coli and promotes RNA polymerase binding and transcription
• Abstracts: RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli. part 2 Expression and characterization of the prtV gene encoding a collagenase from Vibrio parahaemolyticus in Escherichia coli
• Abstracts: Diurnal variation of metabolic responses to short-term fasting in passerine birds during the postbreeding, molting and migratory period. part 2
• Abstracts: A chitin-binding domain in a marine bacterial chitinase and other microbial chitinases: implications for the ecology and evolution of 1,4-beta-glycanases

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.