Characterisation of the gene encoding nitrite reductase and the physiological consequences of its expression in the nondenitrifying Rhizobium " hedysari" strain HCNT1

Article Abstract:

The nirK gene of Rhizobium hedysari HCNT1 encodes nitrite reductase, and the reduction of nitrite causes the production of large concentrations of freely diffusible nitric oxide (NO). The nitrite reductase is structurally similar to the copper containing family of nitrite reductases. HCNT1 fails to reduce or grow anaerobically with nitrate or nitrous oxide. The bacteria is unable to link nitrite reduction to energy conservation, and the amount of NO it produces is much higher than that produced by other denitrifiers.

Research, Physiological aspects, Rhizobium, Microbial enzymes

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Expression of nitrite and nitric oxide reductases in free-living and plant-associated Agrobacterium tumefaciens C58 cells

Article Abstract:

The association with plants affects expression of denitrification genes, the regulation of nitrite and nitric oxide reductases was investigated in Agrobacterium tumefaciens. It was found that the addition of a nitric oxide sythase inhibitor to block nitric oxide generation by the plant prevented gfp expression in infiltrated nitrite reductase mutants, demonstrated that plant-derived nitric oxide can activate nor expression in infiltrated cells.

Nitrites, Agrobacterium tumefaciens, Chemical properties

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Use of a green fluorescent protein-based reporter fusion for detection of nitric oxide produced by denitrifiers

Article Abstract:

This article describes the development of a green fluorescent protein-based assay for the detection of nitric oxide by bacteria. Research describes the fusion of green fluorescent protein, gfp, to nmrS protein from Rhodobacter sphaeroides 2.4.3. Data indicate that this fusion-protein system responds to levels of nitric oxide produced by denitrifiers.

United States, Product information, Methods, Usage, Testing, Denitrification, Fluorescent chemicals, Assaying

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