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Biological sciences

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Characterization and expression of the hydrogenase-encoding gene from Clostridium acetobutylicum P262

Article Abstract:

DNA sequencing of the hydrogenase-encoding gene (hydA) isolated by colony hybridization from Clostridium acetobutylicum P262 reveals the presence of an open reading frame of 1722 bp that codes for a protein with 574 amino acids. C. acetobutylicum hydrogenase product matches Clostridium pasteurianum hydrogenase-1 protein in 67% of its sequence and resembles it in 82% of the sequence. Primer extension experiments reveal that mRNA transcription initiates at the 5' end. Northern blot analysis reveals the translation of the C. acetobutylicum hydrogenase protein product to be from a monocistronic operon.

Genetic aspects, Amino acid sequence, Amino acid sequencing

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A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis

Article Abstract:

A study of the nucleotide sequencing and database analysis of the protein sequence of the region affecting metronidazole sensitivity in E. coli F19 reveals that it contains a regulator gene, regA, which has 40 percent identity to the ccpA gene of B. subtilis. The regA gene inhibited the expression of a C. acetobutylicum gene that allows the starch to degrade into acetone, butanol and ethanol. Analysis of the solvent-producing pathways and acid-producing pathways did not yield the cause of the molecular changes.

Bacillus subtilis, Metronidazole, Amylases

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The glyceraldehyde-3-phosphate dehydrogenase of Clostridium acetobutylicum: isolation and purification of the enzyme, and sequencing and localization of the gap gene within a cluster of other glycolytic genes

Article Abstract:

The purification and isolation of glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum was accomplished by sequential ammonium sulfate precipitation, gel filtration and anion-exchange chromatography. The gap gene, which was cloned and sequenced from C. acetobutylicum DSM 792, was observed to be clustering with other genes of enzymes from the glycolytic pathway. No genetic sequences similar to rho-independent transcription terminators were observed in the intergenic regions.

Genetic research

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