Characterization and transcriptional analysis of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine

Article Abstract:

A single transcript in Pseudomonas syringae encodes the gene cluster for the biosynthesis of coronafacic acid (CFA), which is the polyketide component of the phytotoxin coronatine (COR), and CFA ligase (cfl). The cfl gene promoter directs transcription of all the genes in this operon. The expression of cfl promoter is temperature-dependent and the optimal temperature is 18 degrees celcius. Functional state of a two-component system located within the COR biosynthetic gene cluster regulates the transcription of the cfl/CFA operon.

Genetic aspects, Observations, Bacterial toxins, Promoters (Genetics), Genetic transcription, Transcription (Genetics)

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Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea

Article Abstract:

The effects of environmental, nutritional and host factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea PG4180 were examined. Both growth and coronatine synthesis were affected by carbon source, nutrient levels, amino acid supplements and the presence of complex carbon and nitrogen sources. The results of the study were used to develop a medium which is optimized for coronatine production. A rapid method for the quantitative analysis of coronatine production was also developed.

Toxins

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Molecular and physiological characterization of Pseudomonas syringae pv. tomato and Pseudomonas syringae pv. maculicola strains that produce the phytotoxin coronatine

Article Abstract:

Several group 1 strains of Pseudomonas (P.) syringae pv. tomato and strain 4981 of P. syringae pv. maculicola have the genes for coronatine on a plasmid, while group 2 strains of P. syringae pv. maculicola and tomato tend to have the genes on chromosomes. Researchers analyzed the DNA that produces the plant toxin coronatine in 22 strains of P. syringae. Although the genes were conserved among the different strains, there were differences between those that appeared on plasmids and those that appeared on chromosomes.

Microbial toxins, Chlorosis (Plants)

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