Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis
Article Abstract:
Polymerase chain reaction (PCR) analysis is suggested for detecting genes encoding enterotoxins Bacillus cereus and B. thuringiensis, which cause gastrointestinal diseases including diarrhea. PCR analysis, using primer sets for detecting genes BCET1, BCET3, and BCET4, and protein complexes hemolysin BL and nonhemolytic enterotoxin, proved successful.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Characterization of emetic Bacillus weihenstephanensis, a new cereulide-producing bacterium
Article Abstract:
An objective is presented of investigating the occurrence of the Bacillus cereus group, to characterize the cereulide producers with regard to psychrotolerance and compare them to popular cereulide producers at the pheno- and genotypic levels. Two psychrotolerant Bacillus weihenstephanensis strains, MC67 and MC118 producing cereulide are reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
A rapid PCR-based DNA test for enterotoxic Bacillus cereus
Article Abstract:
The presence of the hblA and bceT hemolysin genes in different strains of Bacillus was determined and correlated with the enterotoxicity in an effort to develop a rapid diagnostic tool to identify Bacillus cereus and determine its enterotoxic potential accurately. Results reveal that the polymerase chain reaction method of screening for the hemolysin HblA complex is an effective and accurate diagnostic tool.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Functional characterization of the proteolytic system of Lactobacillus sanfranciscensis DSM 20451 during growth in sourdough
- Abstracts: Characterization of haemagglutinin activity of Clostridium bolulinum type C and D 165 toxins, and one subcomponent of haemagglutinin (HA1)
- Abstracts: Structure of the ask-asd operon and formation of aspartokinase subunits in the cephamycin producer 'Amycolatopsis lactamdurans'
- Abstracts: Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli
- Abstracts: Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA