Characterization of poly-gamma-glutamate hydrolase encoded by a bacteriophage genome: possible role in phage infection of Bacillus subtilis encapsulated with poly-gamma-glutamate

Article Abstract:

The authors have investigated glutamate capsular polypeptide with gamma-linkage, poly-gamma-glutamate, produced by Bacillus subtilis. The poly-gamma-glutamate hydrolase from the bacteriophage in B. subtilis has been purified, and the results demonstrate that this bacteriophage breaks down poly-gamma-glutamate barrier by means of poly-gamma-glutamate hydrolase.

Enzymes, Bacillus subtilis, Bacteriophages, Bacterial infections, Genomes, Glutamate, Polypeptides, Hydrolases

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Intraspecies polymorphism of Cryptosporidium parvum revealed by PCR-restriction fragment length polymorphism (RFLP) and RFLP-single-strand conformational polymorphism analyses

Article Abstract:

The authors have investigated Cryptosporidium parvum glycoprotein-encoding gene. The use of this gene for identification and gene fingerprinting of C. parvum isolates via PCR-restriction fragment length polymorphism-single-strand conformational polymorphism and PCR-restriction fragment length polymorphism methods is described.

Usage, Glycoproteins, Coccidia, Cryptosporidium

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Identification of cry11-type genes from Bacillus thuringiensis strains and characterization of a novel cry11-type gene

Article Abstract:

Research has been conducted on Bacillus thuringiensis cry11-type genes. The authors describe the use of PCR-restriction fragment length polymorphism method developed for identification of these genes.

China, Bacillus thuringiensis, Methodology, Research methods

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