Characterization of protistan assemblages in the Ross Sea, Antarctica, by denaturing gradient gel electrophoresis

Article Abstract:

A culture independent approach using denaturing gradient gel electrophoresis (DGGE) is applied for obtaining an assessment of the genetic composition and distribution of the protists in various microhabitats like seawater, melt water or ice of the Ross Sea, Antarctica. Sequences representing diatoms, dinoflagellates, ciliates, flagellates and other unidentified eukaryotes were revealed by the sequence analysis of the selected DGGE bands.

Legal issues & crime, Antarctica, Analysis, Usage, Company legal issue, Investigations, Gel electrophoresis, Gene expression, Marine microbiology

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Transport of the harmful bloom alga Aureococcus anophagefferens by oceangoing ships and coastal boats

Article Abstract:

The hypothesis that brown tide is transported long distance via ships' ballast water and on the local scale via recreational boats is tested and described. Aureococcus concentrations in bilge water of recreational boats were determined by quantitative real-time PCR by using a species-specific molecular probe.

Science & research, Goods & services distribution, Regulation and Administration of Transportation Programs, Water Transportation Programs, Research, Transportation, Polymerase chain reaction, Shipping industry, Marine transportation, Phytoplankton

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