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Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

Article Abstract:

An endo-1,3-1,4-beta-glucanase gene from Bacillus macerans has been cloned and expressed in Lactobacillus reuteri. The lactobacilli secreted the beta-glucanase gene which means that B. macerans secretion signals can be recognized by Lactobacillus cells. The beta-glucanase gene could be used to synthesize heterologous peptides of L. reuteri. A new promoter probe vector for L. reuteri can be made from the synthesis of plasmid pNCKH113.

Author: Jenkinson, Howard F., Tannock, Gerald W., Heng, Nicholas C.K.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Bacillus (Bacteria), Bacillus, Bacterial genetics

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Identification of Lactobacillus sakei genes induced during meat fermentation and their role in survival and growth

Article Abstract:

In vivo expression technology (IVET) is used for investigating gene expression of Lactobacillus sakei 23K during meat fermentation. A molecular basis is established that has allowed the investigation of the bacterial properties contributing to the ecological performance of the organism and also influencing the final outcome of sausage fermentation.

Author: Zagorec, Monique, Chaillou, Stephane, Hertel, Christian, Hufner, Eric, Markieton, Tobias, Crutz-Le Coq, Anne-Marie
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
Analysis, Fermentation, Gene expression

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Ecological behavior of Lactobacillus reuteri 100-23 is affected by mutation of the luxS gene

Article Abstract:

The physiological and ecological role for the luxS gene in Lactobacillus reuteri 100-23C is defined by constructing a luxS mutant by insertional mutagenesis. The results show that LuxS influences important ecological attributes of L. reuteri 100-23C, the consequences of which are niche specific.

Author: Hertel, Christian, Tannock, Gerald W., Bello, Fabio Dal, Ghazally, Salina, Water, Jens, Loach, Diane, Brooks, Heather, Cook, Gregory, Surette, Michael, Simmers, Cameron, Bremer, Phil
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
United States, Science & research, Physiological aspects, Ecological genetics

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Subjects list: Research, Genetic aspects, Lactobacillus
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