Comparison of sensitivity of immunofluorescent microscopy to that of a combination of immunofluorescent microscopy and immunomagnetic separation for detection of Cryptosporidium parvum oocysts in adult bovine feces

Article Abstract:

A direct immunofluorescence assay (DFA) was contrasted with an immunomagnetic separation (IMS) assay combined with immunofluorescent microscopy for their ability to detect low concentrations of Cryptosporidium parvum oocysts in adult bovine feces. IMS-DFA resulted in a 2-log-unit increase in sensitivity compared to DFA alone. The higher sensitivity of the IMS-DFA resulted from testing 2 g of fecal material instead of the 13 to 19 mg of fecal material tested in the DFA. The increased sensitivity was not attributable to a higher percent recovery.

Feces, Microbiology

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DNA sequence similarity between California isolates of Cryptosporidium parvum

Article Abstract:

Research was conducted to determine if nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the amplicons could discriminate C parvum isolates derived from different mammalian sources throughout California. A previously set developed set of PCR primers was used for the experiments. Results indicate that there was no sufficient polymorphism within the amplicon to allow for reliable differentiation between C parvum isolates obtained within California.

Analysis, Parasites, DNA sequencers

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Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California

Article Abstract:

A cross-sectional study was conducted on the risk factors and prevalence of Giardia sp cysts and Cryptosporidium parvum oocsyts in populations of feral pigs in western California. Results show that, of the 221 feral pigs studied, 12 were shedding Cryptosporidium oocysts and 17 were shedding Giardia sp cysts. Results also indicate that feral pigs may cause surface water protozoal contamination.

Diseases, Feral swine, Giardia

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