Comparison of the BAX for screening/E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef
Article Abstract:
The performance of the BAX for Screening/Escherichia coli O157:H7 was compared with the performance of the BAX system assay with traditional culture, an immunodiffusion method and enhanced selective agars. Focus was made on the detection of E. coli O157:H7 from ground beef samples injected with fewer than 3 CFU/g of organisms. Results suggest that the BAX for Screening/E. coli O157:H7 assay performed better than the other conventional methods by showing a detection rate of 96.5% compared to only 71.5% and 39% for the immunodiffusion and the best cultural methods, respectively.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Epidemiological typing of Bacillus spp. isolated from food
Article Abstract:
A comparison of biotypes, fatty acid profiles and restriction fragment length polymorphisms of a PCR product identified 51 Bacillus isolates in milk samples and 11 isolates in food samples. Isolates with mesophilic or psychotropic characteristics were distributed into specific fatty acid or PCR-RFLP groups. Fatty acid clusters found predominantly in milk samples suggest that certain dairy plants have plant-specific B. cereus, which are responsible for postpasteurization contamination.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes
Article Abstract:
Polymerase chain reaction (PCR) assays are used in conjunction with a reverse hybridization method in the cloning and subcloning of the 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis in raw milk. Results indicate that all the Brucella strains tested have similar spacer sequences and very high interspecies relatedness. The data also suggest that PCR assays are very useful for detecting bacterial pathogens in raw milk.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Comparison of two methods for detection of Giardia cysts and Cryptosporidium oocysts in water. Waterborne Giardia cysts and Cryptosporidium oocyts in the Yukon, Canada
- Abstracts: Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples
- Abstracts: Use of commercial enzyme immunoassays and immunomagnetic separation system for detecting Escherichia coli 0517 in bovine fecal samples
- Abstracts: Homofermentative production of D- or L-lactate in metabolically engineered Escherichia coli RR1
- Abstracts: Flow cytometric analysis of characteristics of hybridization of species-specific fluorescent oligonucleotide probes to rRNA of marine nanoflagellates