Compatible solutes protect against chaotrope (ethanol)-induced, nonosmotic water stress

Article Abstract:

The germination of conidia of Aspergillus nidulans, a sensitive indicator of water stress in the presence of ethanol is corelated with the intracellular concentration of the compatible solutes glycerol and erythritol, which protect against both osmotic and nonturgor forms of water stress is reported. The conclusion states that the osmoprotectants glycerol and erythritol protect against ethanol induced water stress in A. nidulans.

Industrial organic chemicals, not elsewhere classified, Ethanol, Ethyl Alcohol Manufacturing, Aspergillus, Alcohol, Microbiological research, Alcohol, Denatured

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Three stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water

Article Abstract:

The spatial and compositional structure and complex dynamics of the developing biofilm and its polychlorinated biphenyls (PCB)-degrading activity, leading to the identification of three stages of biofilm development is reported. The biofilm developing from PCB-contaminated soil on PCB droplets displayed a pronounced pattern of maturation not reported before from pure-culture experiments.

Industrial Organic Chemicals, Polychlorinated Biphenyls, Cyclic Crude and Intermediate Manufacturing, Soil microbiology, Microbial mats, Structure

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Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain

Article Abstract:

A new principle for expression of heat-sensitive recombinant proteins in Escherichia coli at temperatures close to 4 degrees C. was experimentally evaluated. The successful use of a chaperone-based E. coli strain to express heat-labile recombinant proteins at temperatures below the theoretical minimum growth temperature of a common E. coli strain (7.5 degrees C.) is reported.

Analysis, Escherichia coli, Recombinant proteins, Temperature effects

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