DNA structure and novel amino and carboxyl termini of the Chlamydia sigma-70 analogue modulate promoter recognition
Article Abstract:
Research was conducted to examine the hypothesis that the DNA structure and novel amino and carboxyl termini of the Chlamydia sigma-70 analogue modulate promoter recognition. An in vivo heterologous transcription system was developed in Escherichia coli by expressing sigma-66 and assaying sigma-66-dependent expression from chlamydial sequences cloned into a chloramphenicol acetyltransferase reporter vector. Results suggest that a single sigma subunit has the capacity to initiate transcription as a primary molecular mechanism of global gene regulation independent of additional Chlamydia-specific transcription factors.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Assessment of mitochondrial membrane potential in yeast cell populations by flow cytometry
Article Abstract:
Research has been conducted on the rhodamine 123 used for the mitochondrial respiratory function evaluation. The optimization and validation of the low-concentration rhodamine 123 straining protocol for the flow-cytometric assessment of mitochondrial membrane potential change in whole yeast cells have been carried out and are reported.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
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Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorB
Article Abstract:
Research has been conducted on the PotB and MOMP porins from Chlamydia trachomatis. The identification of the metabolites which may be obtained from the host has been carried out, and the ability of these metabolites to enter through PorB or MOMP has been investigated.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
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- Abstracts: Occurrence of choline and glycine betaine uptake and metabolism in the family Rhizobiaceae and their roles in osmoprotection
- Abstracts: Structural and functional organization of the Yersinia pestis bacteriocin pesticin gene cluster. The gene cluster directing O-antigen biosynthesis in Yersinia enterocolitica serotype O:8:identification of the genes for mannose and galactose biosynthesis and the gene for the O-antigen polymerase
- Abstracts: Visualization and modelling of the thermal inactivation of bacteria in a model food. New mathematical modeling approach for predicting microbial inactivation by high hydrostatic pressure
- Abstracts: Glutamate decarboxylase genes as a prescreening marker for detection of pathogenic Escherichia coli groups. Multiplex real-time PCR method to identify shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H(sup)- serotype
- Abstracts: Thermal inactivation of susceptible and multiantimicrobial-resistant Salmonella strains grown in the absence or presence of glucose