Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

Article Abstract:

Former primer 63f and reverse primer 1387r were designed to amplify approximately 1,300 bp of a consensus 16S rRNA gene from bacteria. The efficacy and specificity of these two PCR primers were tested by using them to amplify 16 rRNA genes from template DNA taken from a wide range of organisms, including Eubacterium species and organisms from the coryneform and Micrococcus genera. The amplimer pair 63f-1387r was found to be more successful than the amplimer pair 27f-1392r in amplifying the 16S rRNA genes.

Polymerase chain reaction, Bacterial genetics, Ribosomal RNA, Gene amplification

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Molecular evidence for the evolution of metal homeostasis genes by lateral gene transfer in bacteria from the deep terrestrial subsurface

Article Abstract:

Lateral gene transfer (LGT) plays a vital role in increasing the genetic diversity of microorganisms and promoting the spread of fitness-enhancing phenotypes throughout microbial communities. A study is presented in which phylogenetic analyses is used to examine the role of LGT in the evolution of metal homeostasis in subsurface bacteria.

Science & research, Genetic aspects, Bacteria, Biological diversity, Biodiversity, Phylogeny, Homeostasis, Genetic transformation

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