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Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens, an alga causing recurrent brown tides in the Mid-Atlantic United States

Article Abstract:

The authors have investigated Aureococcus anophagefferens causing algal blooms in estuaries on the Mid-Atlantic United States. They discuss the improved method developed for immunodetection of A. anophagefferens which includes the application of monoclonal-antibody technique allowing high reactivity with the target species but low cross-reactivity with other bacteria species.

Author: Gobler, Christopher J., Caron, David A., Dennett, Mark R., Moran, Dawn M., Schaffner, Rebecca A., Lonsdale, Darcy J., Nuzzi, Robert, McLean, Tim I.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
United States, Analysis, Aquatic microbiology, Cloning, Methodology, Research methods, Viral antibodies, Antibodies, Brown algae

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Mutational analysis of the critical bases involved in activation of the areR-regulated sigma (super)54 -dependent promoter in Acinetobacter sp. strain ADP1

Article Abstract:

Research has been conducted on areR gene from Acinetobacter sp. strain ADP1 which regulates areCBA gene expression. The authors describe the use of mutational strategy in creating specific mutations within upstream activator site of the areCBA promoter region, and discuss the role of the overlapping inverted repeat sequences.

Author: Jones, Rheinallt M., Williams, Peter A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
United Kingdom, Gene mutations, Gene mutation, Genetic regulation, Gene expression

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Subjects list: Research, Physiological aspects, Environmental aspects, Genetic aspects, Bacteria, Microbiology, Microbial populations, Microbial ecology
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