Discrimination of psychrotrophic and mesophilic strains of the Bacillus cereus group by PCR targeting of major cold shock protein genes

Article Abstract:

Research was conducted to examine the discrimination of psychrotrophic and mesophilic strains of the Bacillus cereus group by PCR targeting of major cold shock protein genes. Other dairy-associated bacteria were also screened by PCR assay to ensure that the procedure was specific for the detection of bacteria of the B. cereus group at an annealing temperature of 55 degrees C. Results suggest that the PCR assay will substantially reduce the time necessary for screening pasteurized food and dairy products for contamination by psychrotrophic strains of the B. cereus group.

Bacterial proteins, Bacillus cereus, Psychrotrophic organisms, Psychrotrophic microorganisms

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Differentiation of Listeria monocytogenes serovars by using artificial neural network analysis of Fourier-transformed infrared spectra

Article Abstract:

A classification system based on Fourier transform infrared (FTIR) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of Listeria monocytogenes using a reference of database of 106 well-defined strains. The result data indicate that FTIR identification and serotyping constitute a rapid and inexpensive tool which could be sued routinely in food control to gain additional information on the pathogenic potential of strains isolated from the food-processing chain.

Science & research, Usage, Listeria monocytogenes, Fourier transform infrared spectroscopy

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