Doublecortin, a brain-specific gene mutated in human x-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein

Article Abstract:

Research was conducted to investigate the synthesis of a de novo balanced translocation in a patient with X-linked lissencephaly and double cortex syndrome. Cosmids were selected for shotgun sequencing while polymerase chain reactions were performed on a nondenaturing acrylamide gel run. DNA sequencing was also performed in study using generalized techniques via an automated sequencer. Results of the study showed that the translocation characterized a novel predicted protein called Doublecortin.

Proteins, Brain diseases

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Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing

Article Abstract:

Research was conducted to determine the location and orientation of elongation factor G (EF-G) in the ribosome utilizing directed hydroxyl radical probing to understand the mechanism of EF-G-catalyzed translocation. Polymerase chain reaction was utilized to amplify the EF-G gene from the genomic DNA of Escherichia coli. Results showed that two of the rRNA targets were conserved parts of 23S rRNA that played a major role in elongation factor-dependent functions.

Escherichia coli, Ribosomes, Hydroxylases

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