Effect of Bacillus thuringiensis Cry1 toxins in insect hemolymph and their neurotoxicity in brain cells of Lymantria dispar

Article Abstract:

Research has been conducted on the Cry1 Bacillus thuringiensis toxins. The toxicity of these toxins has been investigated via injection in life insects and in vitro on primary cultures of Lymantria brain cells, and the results indicate that upon injection the targets for the Cry1C toxin reside in the insects' body cavity.

Canada, Belgium, Statistical Data Included, Analysis, Cells (Biology), Cells, Toxins, Microbiological research, Toxicity testing, In vitro tests, Toxicity tests

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Effect of insect larval midgut proteases on the activity of Bacillus thuringiensis cry toxins

Article Abstract:

The effect of midgut juice isolated from third-instar Manduca sexta on the activity of Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ca, Cry1Ea, and R233A was studied. Results conclude that it increased the membrane depolarization ability of Cry1Ab with no effect on other mutant.

Science & research, Health aspects, Research, Bacterial toxins, Nucleic acids, Tobacco hornworm, Tobacco hornworms, Nucleic acid isolation

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Effect of Bacillus thuringiensis toxins on the membrane potential of lepidopteran insect midgut cells

Article Abstract:

A novel in vivo technique was utilized to determine the ability of various Bacillus thuringiensis toxins (BtT) to depolarize the apical membranes of the epithelial cells in freshly isolated lepidopteran larval midguts. Quantitative analysis of the electrical membrane potential of BtT-treated Lymantria dyspar larval midgut epithelial cells indicated the role of the toxins in eliciting rapid membrane depolarization. Furthermore, the effects of BtT in the lepidopteran midgut cells indicated the importance of specific binding and pore formation in the midgut brush border membrane.

Electric properties, Moths, Cell membranes, Microbial toxins, Electrophysiology, Lepidoptera, Membrane potentials, Membrane potential

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