Effectiveness of SYTOX Green stain for bacterial viability assessment

Article Abstract:

The SYTOX Green stain is a high-affinity nucleic acid stain developed for use in bacterial viability assessments. It is able to penetrate cells with compromised membranes but is unable to penetrate membranes of live cells. This characteristic makes it a viable dead cell indicator. This stain was then used in the flow cytometric detection of dead Escherichia coli and Salmonella typhimurium within starved populations in order to test its efficacy. Results show that the stain's use is problematic because it underestimated the fraction of dead cells in the population.

Methods, Physiological aspects, Stains and staining (Microscopy), Stains (Microscopy), Microorganisms, Bacteriology

---

Production of a monoclonal antibody specific for the major outer membrane protein of Campylobacter jejuni and characterization of the epitope

Article Abstract:

Monoclonal antibodies (MAbs) are produced that are directed against the major outer membrane protein (MOMP) of Campylobacter jejuni by using recombinant major outer membrane protein (MOMP) as the antigen. The epitope is located in a region that is exposed to the periplasm and MAb5C4 is shown to be a potentially specific and sensitive MAb that is used for the specific detection and identification of Campylobacter jejuni.

Genetic aspects, Campylobacter, Composition, Membrane proteins

---

Rapid quantification of the toxic alga Prymnesium parvum in natural samples by use of a specific monoclonal antibody and solid-phase cytometry

Article Abstract:

The use of a solid-phase cytometer was described to detect and enumerate the toxic alga Prymnesium parvum in natural samples, using a specific monoclonal antibody and indirect immunofluorescence. It was observed that solid-phase cytometer could be used to rapidly enumerate natural P. parvum cells and detection of other toxic algae, with an appropriate antibody or DNA probe.

Algae, Genetic toxicology

Similar abstracts:

• Abstracts: Effects of long-term storage on plasmid stability in Bacillus anthracis. Generation and molecular characterization of new temperature-sensitive plasmids intended for genetic engineering of Pasteurellaceae
• Abstracts: Effects of long-term storage on plasmid stability in Bacillus anthracis. part 2 Plasmid-mediated high-level gentamicin resistance among enteric, bacteria isolated from pet turtles in louisiana
• Abstracts: Numerical taxonomy of gram-negative, nonmotile, nonfermentative bacteria isolated during chilled storage of lamb carcasses
• Abstracts: The identification of two novel ligands of the FGF receptor by a yeast screening method and their activity in Xenopus development
• Abstracts: Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.