Abstracts

Biological sciences

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Efficacy of activated sludge in removing Cryptosporidium parvum oocysts fromsewage

Article Abstract:

The primary clarifier effluent from a wastewater treatment plant was inoculated with Cryptosporidium parvum oocysts and was passed through a laboratory simulation of activated sludge treatment. The treated water was then used to inoculate CD-1 mice to determine the effectiveness of activated sludge treatment in removing C. parvumoocysts. The results showed that activated sludge could remove 80% to 84% of oocysts. However, this rate of removal could still result in infection of mice.These results indicate that procedures other than activated sludge treatment are required to eliminate health risks posed by C. parvum oocysts.

Health aspects, Water, Water treatment, Sewage sludge

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Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat

Article Abstract:

The PCR based random amplified polymorphic DNA analysis uses polymorphism in the 18S rRNA and internal transcribed spacer 1 (ITS) to identify isolates of the pathogenic protozoa Cryptosporidium parvum. The major sequence change is a deletion in a region of the 18S rRNA. The isolates form two subgroups. One subgroup consists of isolates from human patients and the other from a variety of other hosts and experimental animals.

Usage, Identification and classification, Polymerase chain reaction, Genetic polymorphisms, Pathogenic microorganisms, Protozoans, Protozoa, Pathogenic

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Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State

Article Abstract:

Wildlife may be a source of Cryptosporidium in watersheds that supply drinking water to communities. Researchers used PCR analysis on fecal samples and found that white-tailed deer, chipmunks, white-footed mice, skunks, racoons, and muskrats were all carriers of Cryptosporidium parvum.

Disease transmission, Animal health

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