Efficient transformation of Lactobacillus sake by electroporation

Article Abstract:

An effective electroporation method for the transformation of the lactic acid bacteria, Lactobacillus sake, yields up to ten to the seventh power transformants. The method has been developed by studying the effect of electric and other parameters on the transformation of L. sake strain 64F and plasmid pGK12. Short pulses of high voltage or long pulses of low voltage, washing the cells with MgCl2+ -containing solution, and high DNA levels increase transformation. Transformation is unaffected by growth in the presence of cell wall weakening substances.

Methods, Bacterial transformation

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Carbohydrate utilization in Lactobacillus sake

Article Abstract:

The transportation and phosphorylation of sucrose, fructose, glucose, N-acetylglucosamine and mannose occurs through the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in Lactobacillus sake. A chemically defined medium that permits the growth of L. sake and related lactobacilli is used to determine the growth rates on different carbohydrates. The transport of mannose, N-acetylglucosamine and glucose occurs through the PTS permease specific for mannose enzyme II(super Man). PTS-independent permeases transport ribose and gluconate.

Usage, Growth, Bacteria, Carbohydrates, Biological transport

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Disruption of the sole ldhL gene in Lactobacillus sakei prevents the production of both L- and D-lactate

Article Abstract:

The ldhL gene encoding the L(+)-lactate dehydrogenase (L-LDH) of the Lactobacillus sakei was characterized and related to the production of the D- and L-isomers of lactate by the organism's mutant and parent strains. L. sakei is a lactic acid bacterium capable of producing lactate through fermentation of carbohydrates in meat products. It was discovered that the genetic mutation decreased the production of L- and D-lactate indicating that the L-LDH and the racemase were the enzymes responsible for the production of L- and D-lactate, respectively.

Genetic aspects, Lactic acid, Lactate dehydrogenase

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