Engineering of quasi-natural Pseudomonas putida strains for toluene metabolism through an ortho-cleavage degradation pathway
Article Abstract:
A bacterial catalyst for bioconversion of the aromatic hydrocarbon toluene and several alkyl and chloro- and nitro-substituted derivatives into the corresponding benzoates was developed by fully reassembling the upper TOL operon of plasmid pWWO of Pseudomonas putida as a single gene cassette along with its cognate regulatory gene, xylR. To match the corresponding DNA segment to the chromosome of a P. putida strain, a genetic method was used that facilitates the elimination of all recombinant tags carried over from earlier cloning steps and that leaves the natural strain bearing only the relevant DNA segment. The results are discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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phnE and glpT genes enhance utilization of organophosphates in Escherichia coli K-12
Article Abstract:
Organophosphoric compounds (OPCs) belong to a class of pesticides in use around the world. They account for a large number of poisonings in humans and pose hazards for the environment. Thus, microbial degradation of the compounds are of great interest for researchers. In an effort to better understand the metabolic process behind the microbial utilization of OPCs. spontaneous organophosphate utilization mutants of the Escherichia coli K-12 strain was investigated. Two genes responsible for the mutation were then identified using cloning experiments.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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