Evidence of a role for NAD+-glycohydrolase and ADP-ribosyltransferase in growth and differentiation of Streptromyces griseus NRRL B-2682: inhibition by m-aminophenylboronic acid

Article Abstract:

The ADP-ribosyltransferase of Streptomyces griseus NRRL B-2682 is an important enzyme for growth and differentiation of the strain. It may function as a regulator of the activity of major enzymes or developmental proteins by responding to intra- and extracellular conditions. m-Aminophenylboronic acid (APBA) was found to inhibit the germination, mycelial growth and sporulation in a manner dependent on concentration and age. APBA was also found to irreversibly inhibit NAD+-glycohydrolase and ADP-ribosyltransferase activity upon addition to cells or cell extracts.

Cell differentiation, Enzyme inhibitors

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Membrane-bound and extracellular beta-lactamase production with developmental regulation in Streptomyces griseus NRRL B-2682

Article Abstract:

A membrane-bound and an extracellular form of a beta-lactamase were produced from Streptomyces griseus NRRL B-2682. While biochemical characterization experiments showed that the enzyme belongs to the class A group of penicillinase, it was also observed that the enzymes were processed differently. Moreover, the differentiation state of the strain also affects the secretion of the beta-lactamases. The results also showed that a close relationship exists between beta-lactamase secretion and the sporulation process in S. griseus.

Beta lactamases

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Expression analysis of the ssgA gene product, associated with sporulation and cell division in Streptomyces griseus

Article Abstract:

Western analysis with an anti-SsgA serum was used to investigate the possible role of the ssgA gene product (SsgA) in cell division and morphological development of Streptomyces griseus. The close correlation between the SsgA accumulation and the onset of sporulation observed in wild-type cells and the absence of ssgA product in some developmental mutants demonstrated the significant role of the ssgA gene product in sporulation and cell division.

Cell division, Streptomycin, Cell physiology

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