Exploiting genotypic diversity of 2,4-diacetylphloroglucinol-producing Pseudomonas spp.: characterization of superior root-colonizing P. fluorescens strain Q8r1-96

Article Abstract:

Pseudomonas fluorescens strain Q8r1-96 can suppress take-all disease of wheat even after eight successive growth cycles whereas other strains had no activity after five to seven cycles. Biological control efforts may be more successful if scientists use the existing diversity of antagonistic rhizobacteria.

Prevention, Pests, Biological control, Plant diseases, Pseudomonas fluorescens

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Role of ptsP, orfT, and sss recombinase genes in root colonization by Pseudomonas fluorescens Q8r1-96

Article Abstract:

An investigation on three genes, an sss recombinase gene, ptsP, and orfT, which are important in the interaction of Pseudomonas spp. with various hosts, is conducted to determine their contributions to the unusual colonization properties of strain Q8r1-96. The sss recombinase and ptsP gene are found to influence global processes, including phenotypic plasticity and organic nitrogen utilization, respectively.

United States, Science & research, Genetic aspects, Recombinases

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Frequency of antibiotic-producing Pseudomonas spp. in natural environments

Article Abstract:

The synthesis of 2,4-diacetylphloroglucinol (Phl) and phenazine-1-carboxylic acid (PCA) by naturally occurring fluorescent Pseudomonas spp. was analyzed by utilizing specific primers and probes. Detection of Phl and PCA synthesis by Pseudomonas spp. on roots of wheat by specific primers indicated the presence of conserved genes within the biosynthetic loci for Phl and PCA. Furthermore, the primers and probes also allowed the specific and efficient detection of Phl- and phenazine-producing microbial strains from natural environments.

Methods, Analysis, Physiological aspects, Microbiological synthesis, Soil microbiology, Microbial biotechnology

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