Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing

Article Abstract:

A kit method devised for purification of bacterial artificial chromosomes (BACs) could be adapted for effectively preparing individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. A protocol is described for using solid-phase reversible immobilization (SPRI) for rapid, efficient and inexpensive isolation of sequencing-quality DNA of individual large, low-copy number plasmids from gram-negative and gram-positive bacterial strains.

Risk factors, Bacteriophages, Nucleotide sequencing

---

Enantioselective synthesis of S-equol from dihydrodaidzein by a newly isolated anaerobic human intestinal bacterium

Article Abstract:

A chemically synthesized dihydrodaidzein was used as the substrate administered to a newly isolated reshaped, gram-negative, anaerobic bacterium from human feces, which can convert dihydrodaidzein to S-equol. Chiral stationary-phase high-performance liquid chromatography eluted only one metabolite, S-equol, which was produced form a bacterial culture containing a racemic mixture of dihydrodaidzein.

Ligands (Biochemistry), High performance liquid chromatography

---

Epoxide formation on the aromatic B ring flavanone by biphenyl dioxygenase of pseudomonas pseudoalcaligenes KF707

Article Abstract:

The first prokaryotic epoxide formation on the aromatic ring structures by biphenyl dioxygenase is presented. It suggests that different substrate configurations may react differently with high Fe oxo species of biphenyl dioxygenase.

Prokaryotes, Pseudomonas, Biphenyl compounds, Biphenyls (Class of compounds), Chemical properties

Similar abstracts:

• Abstracts: Simultaneous recovery of extracellular and intracellular DNA suitable for molecular studies from Marine sediments
• Abstracts: Diversity and structure of bacterial chemolithotrophic communities in pine forest and agroecosystem soils. Analysis of facultative lithotroph distribution and diversity on volcanic deposits by use of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase
• Abstracts: Spontaneous rDNA copy number variation modulates Sir2 levels and epigenetic gene silencing. The A, B, Gs of silencing
• Abstracts: Epidemiology, relative invasive ability, molecular characterization, and competitive performance of Campylobacter jejuni strains in the chicken gut
• Abstracts: Improvement of oligonucleotide probe design criteria for functional gene microarrays in Environmental applications

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.