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Abstracts » Biological sciences

Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing

Article Abstract:

A kit method devised for purification of bacterial artificial chromosomes (BACs) could be adapted for effectively preparing individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. A protocol is described for using solid-phase reversible immobilization (SPRI) for rapid, efficient and inexpensive isolation of sequencing-quality DNA of individual large, low-copy number plasmids from gram-negative and gram-positive bacterial strains.

Author: Lapidus, Alla, Williams, Laura E., Detter, Chris, Barry, Kerrie, Summers, Anne O.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Risk factors, Bacteriophages, Nucleotide sequencing

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Enantioselective synthesis of S-equol from dihydrodaidzein by a newly isolated anaerobic human intestinal bacterium

Article Abstract:

A chemically synthesized dihydrodaidzein was used as the substrate administered to a newly isolated reshaped, gram-negative, anaerobic bacterium from human feces, which can convert dihydrodaidzein to S-equol. Chiral stationary-phase high-performance liquid chromatography eluted only one metabolite, S-equol, which was produced form a bacterial culture containing a racemic mixture of dihydrodaidzein.

Author: Hor-Gil Hur, Xiu-Ling Wang, Je Hyeon Lee, Ki Tae Kim, Su-II Kim
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Ligands (Biochemistry), High performance liquid chromatography

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Epoxide formation on the aromatic B ring flavanone by biphenyl dioxygenase of pseudomonas pseudoalcaligenes KF707

Article Abstract:

The first prokaryotic epoxide formation on the aromatic ring structures by biphenyl dioxygenase is presented. It suggests that different substrate configurations may react differently with high Fe oxo species of biphenyl dioxygenase.

Author: Jaehong Han, Song-Young Kim, Jihyun Jung, Yoongho Lim, Joong-Hoon Ahn, Su-Il Kim, Hor-Gil Hur
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Prokaryotes, Pseudomonas, Biphenyl compounds, Biphenyls (Class of compounds), Chemical properties

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Subjects list: Research, Analysis, Physiological aspects, Genetic aspects, Gram-negative bacteria
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