Development and application of a most-probable-number-PCR assay to quantify flagellate populations in soil samples
Article Abstract:
Researchers successfully used a most-probably-number PCR assay to measure the soil protozoan Heteromita globosa using a 700-bp sequence from the rRNA small subunit gene as a primer. Most soil protozoa are too small and amoeboid to be counted by microscopy and dilution culturing techniques are labor-intensive and potentially misleading.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Fate of Enterobacter cloacae JP120 and Alcaligenes eutrophus AEO106(pRO101) in soil during water stress: effects on culturability and viability
Article Abstract:
The influence of water stress on the fate of two soil bacteria, Enterobacter cloacae JP120 and Alcaligenes eutrophus AEO106(pRO101) was compared by using selective plating, immunofluorescence microscopy and DNA hybridization. It was shown that A. eutrophus did not tolerate dessication as well as E. cloacae. The different detection methods gave almost the same results for the field-moist soil, but differences were observed in the air-dried soil. A fraction of nonculturable cells of the two organisms was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Degradation of 4-Chloro-2-methylphenoxyacetic acid in top and subsoil is quantitatively linked to the class III tfdA Gene
Article Abstract:
A quantitative real-time PCR method was used to monitor the increase in the concentration of tfdA genes during degradation of the phenoxy acid herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) in sandy topsoil and subsoil over a period of 115 days. Analyses showed a shift in the dominant tfdA population structure occurred in the said period and the tfdA genes responsible for the degradation belonged to class III whereas the tfdA genes in the soil before degradation belonged to class I.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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- Abstracts: Occurrence of Shewanella algae in Danish coastal water and effects of water temperature and culture conditions on its survival
- Abstracts: Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar mucleotide and exopolysaccharide biosynthesis
- Abstracts: Genetic evidence that loss of virulence associated with gacS or gacA mutations in Pseudomonas syringae B728a does not result from effects on alginate production
- Abstracts: Alginate formation in Azotobacter vinelandii UWD during stationary phase and the turnover of poly-beta-hydroxybutyrate
- Abstracts: Prevalence of Campylobacter spp. Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C. area