Development and application of a most-probable-number-PCR assay to quantify flagellate populations in soil samples

Article Abstract:

Researchers successfully used a most-probably-number PCR assay to measure the soil protozoan Heteromita globosa using a 700-bp sequence from the rRNA small subunit gene as a primer. Most soil protozoa are too small and amoeboid to be counted by microscopy and dilution culturing techniques are labor-intensive and potentially misleading.

Usage, Measurement, Polymerase chain reaction, Protozoa, Protozoans

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Fate of Enterobacter cloacae JP120 and Alcaligenes eutrophus AEO106(pRO101) in soil during water stress: effects on culturability and viability

Article Abstract:

The influence of water stress on the fate of two soil bacteria, Enterobacter cloacae JP120 and Alcaligenes eutrophus AEO106(pRO101) was compared by using selective plating, immunofluorescence microscopy and DNA hybridization. It was shown that A. eutrophus did not tolerate dessication as well as E. cloacae. The different detection methods gave almost the same results for the field-moist soil, but differences were observed in the air-dried soil. A fraction of nonculturable cells of the two organisms was demonstrated.

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Degradation of 4-Chloro-2-methylphenoxyacetic acid in top and subsoil is quantitatively linked to the class III tfdA Gene

Article Abstract:

A quantitative real-time PCR method was used to monitor the increase in the concentration of tfdA genes during degradation of the phenoxy acid herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) in sandy topsoil and subsoil over a period of 115 days. Analyses showed a shift in the dominant tfdA population structure occurred in the said period and the tfdA genes responsible for the degradation belonged to class III whereas the tfdA genes in the soil before degradation belonged to class I.

Science & research, Cladistic analysis, Microbial populations

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