Floc stabilization for multiple microscopic techniques

Article Abstract:

A stabilizing correlative microscopic technique characterizes the microbial flocs that are embedded in agarose. These are retained in a porous, resilient medium, which permits the transport, staining and subsampling of the flocculated material directly without any destructive forces. The low melting agarose in conjunction with plankton chambers stabilizes flocculated material. An agarose disc can be subdivided into numerous sections for microscopic analysis.

Analysis, Microbial aggregation, Microscopes

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A gliding bacterium strain inhibits adhesion and motility of another gliding bacterium strain in a marine biofilm

Article Abstract:

A study was conducted to analyze the inhibition of adhesion and motility of a gliding bacterium strain in a marine biofilm by a gliding bacterium strain. Two species of gliding bacteria were determined from members of the genus Cytophaga. Carbohydrate analysis was then carried out on fast protein liquid chromatography-purified material hydrolyzed in HCl at 100 degrees C. Moreover, the effect of the inhibition of other aquatic gliding bacteria was discussed.

Bacteria, Bacterial motility

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Method for collecting air-water interface microbes suitable for subsequent microscopy and molecular analysis in both research and teaching laboratories

Article Abstract:

A new method developed for collecting air-water interface (AWI) microbes and biofilms, which are then used for microscopy and molecular analysis in research and teaching laboratories, is described. The microbe collection method provides a new technique for specific research and teaching applications and can be used in acquiring images and databases of the organisms belonging to the specialized AWI niche.

Science & research

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