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Biological sciences

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Generalized transduction of small Yersinia enterocolitica plasmids

Article Abstract:

Research was conducted to examine the ability of Yersinia enterocolitica plasmids to transduce naturally occurring plasmids. The objective was to investigate phage-mediated gene transfer in Yersinia. Transduction studies were carried out with a temperate phage isolated from a pathogenic Yersinia enterocolitica strain and phage mixtures isolated from sewage. Results demonstrate that the temperate phage and the Yersinia phages from sewage could transduce small plasmids isolated from Y enterocolitica biogroup 1A strains.

Genetic aspects, Plasmids, Transduction, Transduction (Genetics)

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The absence of D-alanine from lipoteichoic acid and wall teichoic acid alters surface charge, enhances autolysis and increases susceptibility to methicillin in Bacillus subtilis

Article Abstract:

The effects of depriving D-alanine ester from lipoteichoic acid and wall teichoic acid in the Bacillus subtilis was analyzed using insertional activations of the genes dlt operon. Results have shown a rise in the the cell walls' negative charge, increased autolysis and susceptibility to methicillin. However, the D-alanine ester has no protective effect against from phagocytosis and degradation in macrophages.

Bacillus subtilis, Bacterial cell walls, Alanine, Methicillin

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Characterization of enterocoliticin, a phage tail-like bacteriocin, and its effect on pathogenic Yersinia enterocolitica strains

Article Abstract:

A bacteriocin from Yersinia enterocolitica 29930 in serogroup O:7,8 can inhibit enteropathogenic strains of Y. enterocolitica from serogroups O:3, O:5,27, and O:9. It forms a channel leading to an efflux of potassium and causes a complete loss of cellular metabolic activity in sensitive strains.

Bacterial toxins

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