Genetics of gamma-irradiation-induced mutations in Arabidopsis thaliana: Large chromosomal deletions can be rescued through the fertilization of diploid eggs
Article Abstract:
Deletion mutagenesis is used for fine genetic mapping of mutations and DNA markets in several plant species. Gamma-irradiated haploid wild-type pollen was used to pollinate diploid and tetraploid multimarker lines of the model higher plant Arabidopsis. Chromosome loss mutations and large deletions were found to be induced in the irradiated pollen, but were eliminated in diploid M1 plants and could be rescued in triploid M1 progeny.
Publication Name: The Journal of Heredity
Subject: Biological sciences
ISSN: 0022-1503
Year: 1999
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Heteroplasmy of mitochondrial DNA in the Ophiruroid Astrobrachion constrictum
Article Abstract:
The presence of mitochondrial heteroplasmy is demonstrated in the cytochrome oxidase 1 (CO1) gene of the brittle star Astrobrachion constrictum. Distinct single-strand conformation polymorphism (SSCP) haplotypes differing by two substitutions were found, and there was evidence for heteroplasmy. The haplotypes were isolated and characterized using polymerase chain reaction cloning, SSCP and sequencing.
Publication Name: The Journal of Heredity
Subject: Biological sciences
ISSN: 0022-1503
Year: 2000
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