Group-specific 16S rRNA hybridization probes for determinative and community structure studies of Butyrivibrio fibrisolvens in the rumen

Article Abstract:

Oligonucleotide probes covering three phylogenetically defined groups of Butyrivibrio spp. were successfully designed and tested. The specificity of each probe was assessed by hybridization to rRNAs from an assortment of B. fibrisolvens isolates as well as additional ruminal and nonruminal bacteria. When RNA was extracted from a culture of OB156, the probe was able to detect target cells at densities as low as 10000 cells/ml. When 10000 or more target cells/ml were added to cattle rumen samples, detectable hybridization signals were obtained with 1000 ng of total RNA loaded onto the nylon membrane.

Bacteria, Bacteriology

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Sequence analysis and characterization of pOM1, a small cryptic plasmid from Butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for butyrivibrios

Article Abstract:

The pOM1 plasmid DNA was cloned and sequenced as a preliminary step for the construction of vector systems for Butyrivibrios. The pOM1 plasmid of Butylrivibrio fibrisolvens exhibited a nucleotide sequence that is composed of two open reading frames namely, ORF1 and ORF2, which mediate self-replication. The ORF1 also encodes a protein that is similar to the plasmid recombination enzyme from gram-positive bacteria. Furthermore, the native Butyrivibrio plasmid can form the basis for a second, independent and compatible family of cloning vectors.

Analysis, Plasmids, Genetic vectors

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Postinoculation protozoan establishment and association patterns of methanogenic archaea on the ovine rumen

Article Abstract:

The evaluation of association patterns between archaea and rumen protozoa by analyzing archaeal 16S rRNA gene clone libraries from ovine rumen inoculated with different protozoa is discussed. Findings reveal the association of different archaeal phylotypes with specific groups of protozoa.

Science & research, Genetic aspects, RNA, Protozoa, Protozoans

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