High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey
Article Abstract:
Kluyveromyces lactis is a lactose-utilizing yeast that can be grown in cottage cheese whey, leading to high-level production of the heterologous protein lysozyme. Two Kluyveromyces lactis strains, K6 and K7, have been utilized in an experiment to express the recombinant human lysozyme. The amounts obtained for lysozyme were considerably higher than those obtained by growing the same strain in defined laboratory settings. In fact, even unsterilized cottage cheese whey could be used as a fermentation medium as long as it was kept refrigerated and utilized reasonably soon after collection.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Association of bacteria and yeasts in hot springs
Article Abstract:
Research was conducted to examine the association of bacteria and yeasts in hot springs. The objective was to investigate the influence of the thermophilic bacterium Bacillus sp strains TB-1 and its extracellular metabolites on the growth of Debaryomyces vanriji at different incubation temperatures. TB-1, TB-2 and TB-3 and Saccharomyces cerevisiae AP2445 were used in the experiment. Results indicate that TB-1 excreted thiamine into the culture broth which not only induced D vanriji growth in mixed culture but also increased the maximal temperature for yeast growth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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A rapid method for screening large numbers of environmental microorganisms for antiviral activity
Article Abstract:
A novel procedure for screening microbial environmental colonies with antiviral activity is presented. Based on close contact between microbial agar cultures and agar-covered virus-infected-cell monolayers, it has several advantages. First, it allows the screening of large numbers of colonies in just a few months. Second, it is easy to execute. Another advantage is that it allows the detection of antiviral activity in colonies which lose their productivity swiftly after their first passage in test media.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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