High-pressure, high-temperature bioreactor for comparing effects of hyperbaric and hydrostatic pressure on bacterial growth
Article Abstract:
The effects of two methods of pressurization were examined on a thermophilic archaebacterium ES4. To accomplish this, a high-pressure reactor system was developed suitable for simultaneous hyperbaric and hyrostatic pressurization at elevated temperatures. At 95 degrees centigrade with 500 atm, the growth rate of ES4 was significantly higher under hydrostatic pressurization. Elevating the temperature also showed higher growth in the hydrostatic system. The system can be useful in assessing the effects of pressure and other parameters on the growth of deep-sea thermophiles.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Cadmium removal by a new strain of Pseudomonas aeruginosa in aerobic culture
Article Abstract:
A fluorescent pseudomonad derived from a deep-sea vent was found to be capable of growing in cadmium concentrations of up to 5mM. Strain CW-96-1 was observed to remove over 99% of the cadmium from the artificial seawater medium containing citrate after 140 hours. Energy dispersive microanalysis show that cadmium removal was achieved by cell wall precipitation. Fatty acid analysis and 16S ribosomal DNA sequencing also reveal that the fluorescent pseudomonad is a Pseudomonas aeruginosa strain.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Pressure-enhanced activity and stability of a hyperthermophilic protease from a deep-sea methanogen
Article Abstract:
A study was conducted on the characteristics of a hyperthermophilic, barophilic protese from the deep-sea methanogen Methanococcus jannaschii. The enzyme was found to exhibit narrow substrate specificity and a 29 kDa molecular mass. Spin labeling showed that the protease's active site geometry is similar to that of various mesophilic proteases. The thermophilic and barophilic properties observed were found to be consistent with those identified in previous Methanococcus jannaschii studies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Alternative strategies for becoming an insider: lessons from the bacterial world. Temporal regulation of Salmonella virulence effector function by proteasome-dependent protein degradation
- Abstracts: Earthworm egg capsules as vectors for the environmental introduction of biodegradative bacteria. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants
- Abstracts: Succession and convergence of biofilm communities in fixed-film reactors treating aromatic hydrocarbons in groundwater
- Abstracts: Biochemical and mutational analysis of a Gingipain-like peptidase activity from Prevotella ruminicola B(sub 1)4 and its role in ammonia production by ruminal bacteria
- Abstracts: Excretion of ammonium by a nifL mutant of Azotobacter vinelandii fixing nitrogen. Availability of glutamate and arginine during acid challenge determines cell density-dependent survival phenotype of Escherichia coli strains