High-pressure, high-temperature bioreactor for comparing effects of hyperbaric and hydrostatic pressure on bacterial growth

Article Abstract:

The effects of two methods of pressurization were examined on a thermophilic archaebacterium ES4. To accomplish this, a high-pressure reactor system was developed suitable for simultaneous hyperbaric and hyrostatic pressurization at elevated temperatures. At 95 degrees centigrade with 500 atm, the growth rate of ES4 was significantly higher under hydrostatic pressurization. Elevating the temperature also showed higher growth in the hydrostatic system. The system can be useful in assessing the effects of pressure and other parameters on the growth of deep-sea thermophiles.

Physiological aspects, Hydrostatic pressure, Hyperbaric oxygenation

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Cadmium removal by a new strain of Pseudomonas aeruginosa in aerobic culture

Article Abstract:

A fluorescent pseudomonad derived from a deep-sea vent was found to be capable of growing in cadmium concentrations of up to 5mM. Strain CW-96-1 was observed to remove over 99% of the cadmium from the artificial seawater medium containing citrate after 140 hours. Energy dispersive microanalysis show that cadmium removal was achieved by cell wall precipitation. Fatty acid analysis and 16S ribosomal DNA sequencing also reveal that the fluorescent pseudomonad is a Pseudomonas aeruginosa strain.

Pseudomonas aeruginosa, Bacteria, Aerobic, Aerobic bacteria, Cadmium

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Pressure-enhanced activity and stability of a hyperthermophilic protease from a deep-sea methanogen

Article Abstract:

A study was conducted on the characteristics of a hyperthermophilic, barophilic protese from the deep-sea methanogen Methanococcus jannaschii. The enzyme was found to exhibit narrow substrate specificity and a 29 kDa molecular mass. Spin labeling showed that the protease's active site geometry is similar to that of various mesophilic proteases. The thermophilic and barophilic properties observed were found to be consistent with those identified in previous Methanococcus jannaschii studies.

Proteases, Methanobacteriaceae, Methanogens

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