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Biological sciences

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Homologs of aflatoxin biosynthesis genes and sequence of aflR in Aspergillus oryzae and Aspergillus sojae

Article Abstract:

The presence and expression of several genes involved in aflatoxin biosynthesis in the parasiticus, oryzae and sojae strains of Aspergillus have been determined. The gene encoding the A. oryzae homolog of aflR, which is the transcriptional regulator of the aflatoxin and sterigmatocystin biosynthesis gene clusters, has also been cloned. The presence of four genes, but not their expression, has been found to be necessary for aflatoxin biosynthesis in oryzae and sojae strains. A. oryzae ATCC 14895 clusters with A. sojae based on the sequence comparison of the aflR genes.

Physiological aspects, Bacterial genetics, Cloning

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The carboxy-terminal portion of the aflatoxin pathway regulatory protein AFLR of Aspergillus parasiticus activates GAL1::lacZ gene expression in Saccharomyces cerevisiae

Article Abstract:

Aflatoxin pathway regulatory protein (AFLR), a DNA-binding protein of 444 amino acids, transactivates the expression of aflatoxin biosynthesis genes in Aspergillus parasiticus and Aspergillus flavus, as well as the sterigmatocystin synthesis genes in Aspergillus nidulans. By fusion of various aflR gene coding regions to the GAL4 DNA-binding coding region, it was found that the AFLR carboxyl terminus contained a region that activated GAL1::lacZ gene expression in Saccharomyces cerevisiae and that the AFLR internal region was required for the activation activity.

Genetic regulation, Saccharomyces, DNA binding proteins

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Regulation of aflR and its product, AflR, associated with aflatoxin biosynthesis

Article Abstract:

The function of the regulatory gene aflR and its product,AflR, in the biosynthesis of aflatoxin in Aspergillus was examined using Western blot and enzyme-linked immunosorbent assay analyses. Findings showed that the aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various conditions such as temperature, carbon source, air supply, nitrogen source and zinc availability. Expression of the omtA aflatoxin biosynthetic pathway structural gene was regulated by the presence of AflR.

Usage, Western immunoblotting, Western blot

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