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Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization

Article Abstract:

Strains of Listeria monocytogenes were identified by DNA colony hybridization based on a cloned hlyA gene and its nearby flanking DNA and the ECL direct labeling system. A modified colony format was used in the evaluation of the sensitivity and specifity of the procedure. Test applications were made with several selective media as its sensitivity was compared with that of a radioactive probe. The viability of the system as an alternative was demonstrated.

Author: Lane, John, Datta, Atin R., Moore, Melissa A., Wentz, Barry A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
Research, Methods, DNA probes

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Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes

Article Abstract:

Generally only a few tests are necessary to classify the bona fide Listeria isolates in the six species of the genus. The Listeria innocua isolate comprises of the members of PrfA, which is the standard accumulation of virulence genes of Listeria monocytogenes.

Author: Johnson, J., Swaminathan, B., Jinneman, K., Stelma, G., Smith, B. G., Lye, D., Messer, J., Ulaszek, J., Evsen, L., Gendel, S., Bennett, R. W., Pruckler, J., Steigerwalt, A., Kathariou S., Yildirim, S., Volokhov, D., Rasooly, A., Chizhikov V., Weidmann, M., Fortes, E., Duvall, R. E., Hitchins, A. D.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Analysis, Microbiology, Virulence (Microbiology)

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L. monocytogenes oligonucleotide probe

Article Abstract:

This correspondence clarifies the confusion regarding the oligonucleotide probe from the article

Author: Swaminathan, B., Weaver, Robert E., Datta, Atin R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
Reports, Genetic aspects, Identification and classification, Nucleic acid probes, Letter to the Editor, Genetic research

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Subjects list: Listeria monocytogenes
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