Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture

Article Abstract:

Researchers report the discovery of benzylsuccinate synthase activity in a methanogenic culture. Until this report, this newly discovered enzyme had only been described in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria. The enzyme attaches the methyl carbon of toluene to the double bond of fumarate to form benzylsuccinate.

Biodegradation, Microbial enzymes, Methanobacteriaceae, Methanogens

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Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium

Article Abstract:

A study was conducted to identify benzylsuccinate synthase (BSS) gene sequences in a highly enriched toluene degrading methanogenic consortium. The differential transcription of bssA-2, combined with the bioinformatic analysis of the sequence provides strong evidence that bssA-2 encodes an enzyme that shares the same catalytic function as those encoded by BssA sequences from the nitrate- and iron-reducing microorganisms.

Analysis, Physiological aspects, Genetic aspects, Polymerase chain reaction, Gene expression, Anaerobic bacteria

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Benzylsuccinate formation as a means of anaerobic toluene activation by sulfate-reducing strain PRTOL1

Article Abstract:

Microbiological research using permeabilized, toluene-grown microbial cells of sulfate-reducing strain PRT0L1 suggests that benzylsuccinate formation from toluene-fumarate catalysis occurs in microorganisms other than Proteobacteria denitrifiers. Anaerobic toluene activation by fumarate addition occurs in widely differing bacterial groups.

Microorganisms, Toluene

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