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Biological sciences

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Identification of Pasteurella multocida tryptophan synthase beta-subunit by antisera against strain P1059

Article Abstract:

Pasteurella multocida strain P1059 causes fowl cholera in chickens and turkeys. In vivo-expressed antigenic proteins of the strain are cloned and expressed to identify potential virulence factors. Some of the clones are reacted with chicken antiserum directed against the strain. Particular emphasis is given a 43 kDa protein because of its high expression, making it suitable for examination by SDS-PAGE and immunoblot analysis. It is shown that the 43 kDa protein has the potential to identify virulence factors in P. multocida strain P1059.

Bacterial genetics, Tryptophan

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Acyl carrier protein of Azospirillum brasilense: properties of the purified protein and sequencing of the corresponding gene, acpP

Article Abstract:

A method based on reverse genetics that generated the full-length sequence of the acyl carrier protein (ACP) gene, the gene encoding ACP of Azospirillum brasilense, is presented. It involved the polymerase chain reaction technique with a primer designed from the N-terminal amino acid sequence of the purified ACP and another designed from the highly conserved amino acid sequence of bacterial ACPs. Cloning and sequencing of the inverse PCR products with the acpP region afforded the generation of the nucleotide sequence.

Carrier proteins, Transport proteins

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The 32 kDa major outer-membrane protein of Pasteurella multocida capsular serotype D

Article Abstract:

A 32 kDa major outer-membrane protein of Pasteurella multocida serotype D is purified and characterized. Faint high molecular mass bands in the purified protein may be due its insufficient solubilization in the sample buffer. Its amino acid composition suggests that the protein is a peripheral membrane protein. The protein is compared with other major outer-membrane proteins of P. multocida.

Gram-negative bacteria, Membrane proteins

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