Identification of a complex between centrin and heat shock proteins in CSF-arrested Xenopus oocytes and dissociation of the complex following Oocyte activation

Article Abstract:

The study identifies a centrin/hsp complex in CSF-arrested Xenopus oocytes which dissociates with treatment activating the oocyte for embryological development. It also proposes a model for centrin sequestration prior to centrosome assembly, which is in keeping with hsp and chaperon functions in other systems. The probability of the usefulness of complex formation with the molecular chaperones to enable maintain oocyte protein stores in a quiescent state before their recruitment into particular fates in the developing embryo is also discussed.

Heat shock proteins, Xenopus

---

Molecular cloning and immunological analysis of goldfish cyclin A during oocyte maturation

Article Abstract:

Research indicates different roles for cyclin A-cdc2 kinase and cyclin B-cdc2 kinase during oocyte maturation. Cyclin A cDNA clones were isolated from a library of goldfish oocyte cDNA. Monoclonal antibody from goldfish cyclin A recognized a 47-kDa protein that was not present after the egg was activated. Cyclin A was found in immature oocytes, unlike goldfish cyclin B, and did not show significant changes in protein level during maturation. In mature oocytes, goldfish cyclin A was associated with cdc2 kinase but not with cdk2.

Cloning, Cell cycle, Protein kinases

---

Molecular mechanisms of the activation of maturation-promoting factor during goldfish oocyte maturation

Article Abstract:

A study of maturation-promoting factor (MPF) activation during the maturation of goldfish oocytes reveals that phosphorylation and dephosphorylation of the catalytic subunit of MPF, cdc2, by Thr161 and Tyr15, respectively, affect MPF activation. The resultant structure of activated MPF, in which cdc2 is bound to a regulatory subunit cyclin B and is phosphorylated on Thr161, is independent of the mechanism of activation.

Analysis, Genetic aspects, Phosphorylation

Similar abstracts:

• Abstracts: The ihog cell-surface proteins bind hedgehog and mediate pathway activation. By binding SIRPalpha or calreticulin/CD91, lung collectins act as dual function surveillance molecules to suppress or enhance inflammation
• Abstracts: Initiation of anterior head-specific gene expression in uncommitted ectoderm of Xenopus laevis by ammonium chloride
• Abstracts: Synthesis and selection of de novo proteins that bind and impede cellular functions of an essential mycobacterial protein
• Abstracts: Synthesis and selection of de novo proteins that bind and impede cellular functions of an essential mycobacterial protein. part 2
• Abstracts: Detection and identification of fungi intimately associated with the brown seaweed Fucus serratus. Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.