Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain

Article Abstract:

The enzyme activity and metabolic fluxes in a wild-type and recombinant strain of Aspergillus oryzae have been studied. Citrate synthase and isocitrate dehydrogenase activities were detected only in the mitochondria while glucose-6-phosphate dehydrogenase and glutamate dehydrogenase were observed only in the cytosol. Isocitrate dehydrogenase, glutamate oxaloacetate transaminase, malate dehydrogenase and glutamate dehydrogenase activites were seen in both the mitochondria and cytosol. The flux through the pentose phosphate pathway increased with increasing specific growth rate and was 15% to 26% higher for the wild-type than for the recombinant strain.

Enzymes, Enzyme kinetics, Enzyme structure-activity relationships

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Glucose control in Saccharomyces cerevisiae: the role of MIG1 in metabolic functions

Article Abstract:

The regulatory protein MIG1 plays an important role in the glucose control in Saccharomyces cerevisiae, specifically the signalling cascade of glucose repression. MIG1 controls a variety of metabolic functions such as the metabolism of disaccharides and trisaccharides, galactose metabolism, metabolism of glucose, mannose and fructose, trehalose metabolism and glycerol influx/efflux, gluconeogenesis and glyoxylate shunt, fermentative functions, tricarboxylic acid cycle and respirative functions.

Saccharomyces, Glucose metabolism

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Induction and repression of alpha-amylase production in batch and continuous cultures of Aspergillus oryzae

Article Abstract:

A study on a wild-type and two recombinant strains of Aspergillus oryzae shows that maltose increases alpha-amylase production. Increase in glucose concentration decreases alpha-amylase production. Alpha-amylase formation and accumulation in the cells of the recombinant strains is more than that in the wild-type strain. The recombinant strains also form an unglycosylated form of alpha-amylase which the wild-strain does not. Alpha-amylase production both inside and outside the cells is studied.

Research, Amylases, Glucose

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