Impact of growth in benzoate and m-toluate liquid media on culturability of Pseudomonas putida on benzoate and m-toluate plates

Article Abstract:

Expression of the TOL plasmid may render Pseudomonas putida incapable of growth on benzoate or m-toluate plates, despite having grown in continuous culture on benzoate or m-toluate. After growth in carbon-limited continuous culture, bacteria may have to adapt to the carbon source in solid media and to the increases in potentially inhibitory substrates and intermediates. TOL plasmids may hinder this adaptation. This phenomenon may result in underestimation of viable cell numbers using plate counts.

Plasmids, Pseudomonas putida

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Heat-induced expression and chemically induced expression of the Escherichia coli stress protein HtpG are affected by the growth environment

Article Abstract:

The production of heat-shock protein during stress in E. coli increases when many amino acids and proteins are present in the medium. On the other hand, a simple glucose mineral medium results in normal or even decreased production of heat-shock protein during stress.

Escherichia coli, Heat shock proteins

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