Impaired secretion of a hydrophobic cutinase by Saccharomyces cerevisiae correlates with an increased association with immunoglobulin heavy-chain binding protein (BiP)

Article Abstract:

Research was performed to identify the cause of the low level of secretion of a hydrophobic mutant cutinase by Saccharomyces cerevisiae using pulse-chase experiments, immunogold labelling and electron microscopy. Results revealed a colocalization of hydrophobic mutant cutinase with immunoglobulin heavy-chain binding protein (BiP) in the endoplasmic reticulum. Furthermore, wild-type cutinase expression did not lead to higher levels of molecular chaperone BiP but BiP levels were heightened by increased induction of the hydrophobic mutant cutinase.

Carrier proteins, Transport proteins, Immunoglobulins

---

Expression and secretion of defined cutinase variants by Aspergillus awamori

Article Abstract:

Research was conducted to determine whether the cutinase variants from Fusariium solani pisi can be expressed successfully by the filamentous fungus Aspergillus awamori. Results demonstrate rapid extracellular degradation of cutinase occurred due to the single L51S substitution. The overexpression of the A. awamori gene encoding the chaperone BiP in the cutinase-producing A. awamori strains had no substantial impact on the secretion efficiency of the cutinases.

Genetic aspects, Aspergillus, Secretion, Gene expression, Mutagenesis, Fusarium

---

Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts

Article Abstract:

Researchers were able to increase the secretion of an enzyme by Saccharomyces cerevisiae five-fold by putting an N-glycosylation consensus sequence in the N-terminal region. This technique could be used to enhance the preferential secretion of a particular protein for industrial purposes.

Microbial genetic engineering, Industrial microbiology

Similar abstracts:

• Abstracts: Cell surface expression of bacterial esterase A by Saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response
• Abstracts: Premature truncation of a novel protein, RD3, exhibiting subnuclear localization is associated with retinal degeneration
• Abstracts: The Cdc25 protein of Saccharomyces cerevisiae is required for normal glucose transport. Nitrogen-regulated transcription and enzyme activities in continuous cultures of Saccharomyces cerevisiae
• Abstracts: Isolation of an IHF-deficient mutant of a Pseudomonas aeruginosa mucoid isolate and evaluation of the role of IHF in algD gene expression
• Abstracts: Stimulation of strontium accumulation in linoleate-enriched Saccharomyces cerevisiae is a result of reduced Sr2+ efflux

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.