Abstracts

Biological sciences

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In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy

Article Abstract:

Two new oligonucleotide probes that are designed on the basis of a comparative sequence analysis are complementary to the 16S rRNA region of Legionellaceae. Dot-blot or whole-cell hybridization helps evaluate their probe-specificities against 14 Legionella pneumophila serogroups, 22 Legionella spp. and 72 non-legionellae reference strains. Under optimized conditions, the LEG226 probe hybridizes to 71% of the Legionella strains examined, while the LEG705 strain hybridizes to 90% of them. In situ hybridization helps identify Legionella cells in model biofilms and study the three-dimensional distribution of L. pneumophila in the ciliated protozoan Tetrahymena pyriformis cells, when used in conjunction with confocal laser scanning microscopy.

Research, Evaluation, Observations, Nucleic acid hybridization, Nucleic acid probes, Bacteria, Pathogenic, Pathogenic bacteria

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In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes

Article Abstract:

The applicability of using rRNA-targeted oligonucleotide probes for the in situ identification of bacteria in drinking water was investigated. The results showed that both planktonic and surface-associated bacteria could be detected with the probes at a limit of around 10(super 3) to 10(super 4) ribosomes per cell. In addition, the percentage of positive cells were significantly higher at the surfaces than in the planktonic phase, indicating that surface-attached cells are more active than free-living cells.

Usage, Water, Aquatic microbiology, DNA probes, Marine bacteria

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Isolation of new bacterial species from drinking water biofilms and proof of their in situ dominance with highly specific 16S rRNA probes

Article Abstract:

The dominant bacterial populations in drinking water biofilms in a municipal drinking water distribution system in Berlin, Germany, are identified. Specific oligonucleotide probes were employed in the in situ investigations. Dominant bacterial species were isolated and characterized phylogenetically. In situ probing of bacteria in their natural environment will provide information regarding phylogenetic identity and in situ distribution correlation.

Drinking water, Contamination

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